Deleted
Sample Discontinued
Experience: None
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Part:BBa_K2269026

Designed by: Jose Alvaro Ballesteros Gonzalez   Group: iGEM17_Valencia_UPV   (2017-09-17)


Tnos:Luc:Assembly Reporter RL:YFP:Tnos

Transcriptional unit composed of the 35s inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL). In normal basis, a luciferase will be expressing and only when RDF appears, YFP will be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2692
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2692
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2692
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2692
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3568


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Parameters
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